MPG Antibody

N-Methylpurine-DNA glycosylase is encoded by the MPG gene, mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29) . The completely characterized human gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of MPG to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of MPG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the MPG-null animals.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P29372

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human MPG (NKSFDQRDLAQDEAVW) was used as the immunogen for this MPG antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the MPG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MPG antibody is available for research use only.

Storage Conditions

After reconstitution, the MPG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MPG antibody may be required due to differences in protocols and secondary/substrate sensitivity.