BiP Antibody / GRP78 / HSPA5

HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum.BiP is an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome. Shen et al. (2002) concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi. The findings ofShen et al. (2002) demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

P11021

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human GRP78/BiP (EWLESHQDADIEDFK) was used as the immunogen for this BiP antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IHC-F, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the BiP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BiP antibody is available for research use only.

Storage Conditions

After reconstitution, the BiP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the BiP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of BiP antibody and Lane 1: rat testis; 2: A549; 3: MCF-7; 4: HeLa cell lysate. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.