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TFPI Antibody / Tissue factor pathway inhibitor

Tissue factor pathway inhibitor, also known as EPI, LACI, TFI and TFPT1 is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa) . While Xa is inhibited, the Xa-TFPI complex can subsequently also inhibit the FVIIa-tissue factor complex. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM. The gene is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI has a relative molecular mass of 34,000 to 40,000 depending on the degree of proteolysis of the C-terminal region.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P10646

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 261-280 (QECLRACKKGFIQRISKGGL-human) were used as the immunogen for this TFPI antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the TFPI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TFPI antibody is available for research use only.

Storage Conditions

After reconstitution, the TFPI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the TFPI antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of TFPI antibody and SMMC-7721 lysate; Predicted/observed molecular weight: 35/40-50kDa.

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