Troponin C Antibody / TNNC1

Troponin C, also called TNNC1, and TNC, is a protein that in humans is encoded by the TNNC1 gene. Using a monochromosomal hybrid panel, the TNNC1 gene is mapped to chromosome 3; subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P63316

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human cardiac Troponin C (MDDIYKAAVEQLTEEQKN) was used as the immunogen for this Troponin C antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Troponin C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Troponin C antibody is available for research use only.

Storage Conditions

After reconstitution, the Troponin C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Troponin C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Troponin C antibody and rat skeletal muscle tissue lysate. Predicted molecular weight: ~18 kDa.

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