IL-4 Antibody

Interleukin-4, also knowns as a B-cell stimulatory factor 1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P05112

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the middle region of human Interleukin-4 (AATVLRQFYSHHEKDTR) was used as the immunogen for this IL-4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the IL-4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IL-4 antibody is available for research use only.

Storage Conditions

After reconstitution, the IL-4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Secreted

Image Legend

Western blot testing of IL-4 antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: human CCRF-CEM cell lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.