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IL-3 Antibody

Interleukin-3 is a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL-3 is tandemly arrayed on the long arm of chromosome 5.5.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P08700

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the N-terminus of human Interleukin-3 (KDGDWNEFRRKLTFYLKTLENA) was used as the immunogen for this IL-3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the IL-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IL-3 antibody is available for research use only.

Storage Conditions

After reconstitution, the IL-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Variations in secondary/substrate sensitivities and test protocols may require the IL-3 antibody to be titrated for optimal performance.

Image Legend

Western blot testing of IL-3 antibody and Lane 1: Recombinant human IL-3 protein 10ng; 2: 5ng; 3: 2.5ng

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