Caspase-8 Antibody (large subunit)

The human CASP8 gene, whose product is also known as Caspase-8 and FLICE, encodes an interleukin-1beta converting enzyme (ICE) -related cysteine protease that is activated by the engagement of several different death receptors. It is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex (DISC) . This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to CASP8. Caspase-8 deficiency in humans is compatible with normal development and shows that the enzyme has a postnatal role in immune activation of naive lymphocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL (1), IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q14790

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 257-277 (NNHNFAKAREKVPKLHSIRDR-human) was used as the immunogen for this Caspase-8 antibody. This sequence is from the large subunit.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Caspase-8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-8 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Image Legend

Western blot testing of Caspase-8 antibody (0.5ug/ml) and Lane 1: human A549; 2: (h) HeLa lysate. Predicted molecular weight: ~55 kDa (pro), ~40 kDa (large + small subunit), ~20 kDa (large subunit) .