COX-2 Antibody / PTGS2

Cyclooxygenase (COX) is the key enzyme in conversion of arachidonic acid to PGs. The COX2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P35354

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Amino acids 589-604 (DDINPTVLLKERSTEL-human) were used as the immunogen for this COX-2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized COX2 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This COX-2 antibody is available for research use only.

Storage Conditions

The lyophilized COX-2 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the COX-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE differentiated adenocarcinoma in human appendix tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.