SDHA Antibody

Succinate dehydrogenase complex, subunit a, flavoprotein is a protein that in humans is encoded by the SDHA gene. The gene that codes for the protein is nuclear, even though the protein is located in the inner membrane of the mitochondria. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant protein more susceptible to proteolysis. The findings indicated that SDHA, like other SDH subunits, can act as a tumor suppressor gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P31040

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human SDHA (NMEEDNWRWHFYDTVK) was used as the immunogen for this SDHA antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the SDHA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SDHA antibody is available for research use only.

Storage Conditions

After reconstitution, the SDHA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the SDHA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm (Mitochondria)

Image Legend

Western blot testing of 1) rat brain, 2) rat kidney, 3) rat spleen, 4) rat liver, 5) mouse brain, 6) mouse kidney and 7) mouse liver tissue lysate with SDHA antibody. Predicted molecular weight: 57-73 kDa (multiple isoforms) .