RFC1 Antibody / Replication factor C subunit 1
Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40 (SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P35251
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Limitations
This RFC1 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Location
Nuclear
Image Legend
Documents
Frequently Asked Questions
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