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MCM2 Antibody

MCM2 is a human nuclear protein that plays an important role in onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

P97310

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids 884-904 (DLFKFNKFSRDLKRKLILQQF-mouse) were used as the immunogen for this MCM2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized MCM2 antibody can be stored at 4oC to -20oC. After reconstituion, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MCM2 antibody is available for research use only.

Storage Conditions

The lyophilized MCM2 antibody can be stored at 4°C to -20°C. After reconstituion, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the MCM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC-P: MCM2 antibody testing of rat intestinal lymphocyte tissue

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