ALDH1A1 Antibody / Acetaldehyde dehydrogenase 1

Aldehyde dehydrogenase 1 family, member A1, also called ALDH1, RALDH1 or ALDH, is an enzyme that in humans is encoded by the ALDH1A1 gene. And this protein belongs to the aldehyde dehydrogenases family of proteins. The gene is mapped on 9q21.13. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. It is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. Retinaldehyde is generated by ADH1 from retinol, and its concentration is determined in large part by its subsequent catabolism by ALDH1A1 to retinoic acid.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P00352

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 283-303 (DLDNAVEFAHHGVFYHQGQCC-human) were used as the immunogen for this ALDH1A1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the ALDH1A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ALDH1A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the ALDH1A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the ALDH1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human A549 cells with ALDH1A1 antibody (blue) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.