SLC10A1 Antibody
SLC10A1 (Solute carrier family 10 (sodium/bile acid cotransporter family), member 1), also called NTCP or NTCP1, is found in the basolateral membranes of hepatocytes, which is also involved in the reabsorption of bile acids. The SLC10A1 gene is mapped on 14q24.2 and contains 5 exons and spans about 23 kb. Like other sodium/bile acid transporters, SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane. Injection of in vitro transcribed NTCP cRNA into Xenopus oocytes resulted in the expression of Na (+) -dependent taurocholate uptake. NTCP-mediated taurocholate uptake in oocytes was inhibited by all major bile acid derivatives and by bromosulfophthalein.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
Q14973
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 141-154 (YSRGIYDGDLKDKV-human) were used as the immunogen for this SLC10A1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
The lyophilized SLC10A1 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SLC10A1 antibody is available for research use only.
Storage Conditions
The lyophilized SLC10A1 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Product Datasheet
https://www.nsjbio.com/tds-pdf/slc10a1-antibody-r30755
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the SLC10A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Membrane
Image Legend
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