Monoamine Oxidase B Antibody

Monoamine oxidase B is a protein that in humans is encoded by the MAOB gene. It is a member of the flavin monoamine oxidase family. MAOB catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. Like MAOA, it also degrades dopamine. MAOB is involved in the breakdown of dopamine, a neurotransmitter implicated in reinforcing and motivating behaviors as well as movement. MAOB inhibition is, therefore, associated with enhanced activity of dopamine, as well as with decreased production of hydrogen peroxide, a source of reactive oxygen species.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q8BW75

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of mouse Monoamine Oxidase B (RTYTIRNKNVKYVDL) was used as the immunogen for this Monoamine Oxidase B antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Monoamine Oxidase B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Monoamine Oxidase B antibody is available for research use only.

Storage Conditions

After reconstitution, the Monoamine Oxidase B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Monoamine Oxidase B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Monoamine Oxidase B antibody and Lane 1: mouse liver; 2: (m) lung; 3: rat kidney; 4: (r) brain; 5: (r) liver; 6: (r) lung tissue lysate