5HT1A Receptor Antibody

5HT1AR is a subtype of 5HTR that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. The HTR1A gene is located at 5q12.3. The decreases in 5HT1AR densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5HT1ARs has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. In the dorsal raphe nucleus they are co-localized with neurokinin 1 (NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P08908

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An amino acid sequence from the C-terminus of human 5HT1A Receptor (NKDFQNAFKKIIKCKFCRQ) was used as the immunogen for this 5HT1A Receptor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the 5HT1A Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This 5HT1A Receptor antibody is available for research use only.

Storage Conditions

After reconstitution, the 5HT1A Receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the 5HT1A Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell membrane

Image Legend

IHC staining of FFPE human lung cancer tissue with 5HT1A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.