Ku80 Antibody / XRCC5

XRCC5 (X-ray Repair, Complementing Defective, In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining (NHEJ) pathway. Human colon cancer cells heterozygous for Ku80 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku80 allele results in cells with a drastically reduced doubling time. The locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in the protein is associated with cancer in patients of varying radiosensitivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P13010

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Ku80 (KFSEEQRFNNFLKAL) was used as the immunogen for this Ku80 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Ku80 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ku80 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ku80 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Ku80 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC-P: Ku80 antibody testing of human breast cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.