Lamin B2 Antibody

Lamin B2, also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. Model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2, was generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

Q03252

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 580-597 (EEDLFHQQGDPRTTSRGC) were used as the immunogen for this Lamin B2 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% thimerosal and 0.05% sodium azide

Reconstitution

The lyophilized Lamin B2 antibody can be stored at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Lamin B2 antibody is available for research use only.

Storage Conditions

The lyophilized Lamin B2 antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the Lamin B2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC staining of FFPE rat intestinal tissue with Lamin B2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.