TIM-1 Antibody (KIM-1)
T-cell immunoglobulin and mucin domain-containing protein 1 (TIM-1) or Kidney injury molecule 1 (KIM-1), also known as HAVCR1 and HAVCR, is a protein that in humans is encoded by the KIM1 gene. Biochemical, mutational, and cell adhesion analyses confirm that TIM-1 is capable of homophilic interactions. The features identified in murine TIM1 is conserved in human protein. The protein is a receptor for virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse TIM-1, expression was seen after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression during mouse T-cell differentiation leads to production of the Th2-type cytokine IL4, but not the Th1-type cytokine Ifng.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
O54947
Host
Rabbit
Reactivity
Rat
Immunogen
Amino acids 289-307 (HPRAEDNIYIIEDRSRGAE-rat) were used as the immunogen for this TIM-1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
The lyophilized TIM-1 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TIM-1 antibody is available for research use only.
Storage Conditions
The lyophilized TIM-1 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the TIM-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE rat testis tissue with TIM-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
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