Kallikrein-1 Antibody

Kallikrein-1, also called KLK1 and KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. It is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. Mice lacking the protein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P06870

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the middle region of human Kallikrein-1 (NDECKKAHVQKVTDFM) was used as the immunogen for this Kallikrein-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Kallikrein-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Kallikrein-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Kallikrein-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Kallikrein-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.