IKKa Antibody / IKK alpha

IKBKA, also called CHUK, NFKBIKA, IKKA, IKK1, I-KAPPA-B, is a protein kinase that in humans is encoded by the CHUK gene. It is a member of the protein kinase superfamily. The protein contains a serine/threonine kinase domain. IKKa is expressed in a broad array of tissues and high degree of conservation across species. By FISH, the IKBKA gene is mapped to chromosome 10q24. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. IKKa is part of the I kappa B kinase complex that plays an important role in regulating the NF-kappa B transcription factor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

O15111

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human IKK alpha (NEEQGNSMMNLDWSWLTE) was used as the immunogen for this IKKa antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the IKKa antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IKKa antibody is available for research use only.

Storage Conditions

After reconstitution, the IKKa antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the IKKa antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Flow cytometry testing of fixed and permeabilized human Raji cells with IKKa antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= IKKa antibody.