HSD17B1 Antibody

17-beta-Hydroxysteroid dehydrogenas I, also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase, is an enzyme that is responsible for the interconversion of estrone (E1) and estradiol (E2) as well as the interconversion of androstenedione and testosterone. HSD17B1 gene expression is detected in 34 of 42 (81%) adenomas in all tumor subtypes; HSD17B2 mRNA is detected in 18 of 42 (43%) adenomas but not in prolactinomas. Human estrogenic HSD17B1 is an NADP (H) -preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP (H) over NAD (H) for oxidation and reduction, respectively, as demonstrated by kinetic studies. All four isoforms are variably expressed in human anterior pituitary adenomas, which also show HSD17B enzyme activity, suggesting that it may play an important role in regulating the local cellular levels of estradiol.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

P14061

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids 29-43 (QSFKVYATLRDLKTQ-human) were used as the immunogen for this HSD17B1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the HSD17B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HSD17B1 antibody is available for research use only.

Storage Conditions

After reconstitution, the HSD17B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the HSD17B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.