PAK1 Antibody

Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activated kinases, include PAK1, -2, -3 and -4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q13153

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1-14 (MSNNGLDIQDKPPA-human) were used as the immunogen for this PAK1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the PAK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PAK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PAK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PAK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of PAK1 antibody and Lane 1: rat testis; 2: rat spleen; 3: SW620; 4: MCF-7; 5: CEM; 6: HT1080; Expected molecular weight 60~70kDa.