LIF Antibody / Leukemia inhibitory factor

LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. It is produced in high amounts by the human endometrium and the trophoblast itself, and its receptors are present on cytotrophoblast cells. LIF could thus play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P09056

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of mouse LIF (DKEAFQRKKLGCQLLGTYKQ) was used as the immunogen for this LIF antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the LIF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LIF antibody is available for research use only.

Storage Conditions

After reconstitution, the LIF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the LIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of LIF antibody and Lane 1: rat intestine; 2: (r) brain; 3: (r) spleen; 4: (r) thymus; 5: mouse brain; 6: (m) kidney; 7: (m) liver. The 180 amino acid protein is heavily glycosylated and is routinely visualized from 32~62 KD.