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Cystathionase Antibody / Cystathionine gamma lyase

Selected Variant

R30633

Size: 100 µg

Cystathionine gamma lyase (or Cystathionase) is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P32929

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Cystathionine gamma lyase (FYIKGTLQHAEIFLKN) was used as the immunogen for this Cystathionase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Cystathionase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cystathionase antibody is available for research use only.

Storage Conditions

After reconstitution, the Cystathionase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Cystathionase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with Cystathionase antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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