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IKK gamma Antibody

IKBKG (Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma) is also known as NEMO. The IKK complex is composed of IKKalpha, IKKbeta, and 2 other polypeptides of 50 and 52 kD which are differentially processed forms of a third subunit, IKK gamma. The 419-amino acid IKK gamma protein is composed of several potential coiled-coil motifs and a leucine zipper motif. It interacts with IKK2 (IKKbeta), but not with IKK1 (IKKalpha) . IKK-gamma forms dimers and trimers and interacts preferentially with IKKbeta.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

O88522

Host

Rabbit

Reactivity

Mouse

Immunogen

An amino acid sequence from the N-terminus of mouse IKKg (MNKHPWKNQLSEMVQ) was used as the immunogen for this IKK gamma antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the IKK gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IKK gamma antibody is available for research use only.

Storage Conditions

After reconstitution, the IKK gamma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the IKK gamma antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of IKK gamma antibody and Lane 1: mouse liver; 2: mouse brain; 3: mouse ovary tissue lysate. The protein is visualized from 37~60 kDa.

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