Collagen IV Antibody / COL4A2 / Canstatin fragment

Canstatin, a cleavage product corresponding to the collagen alpha 2 (IV) NC1 domain, is one of the six subunits of Collagen IV and possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P08572

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Collagen IV COL4A2 (amino acids 1697-1712: LIRTHISRCQVCMKNL) was used as the immunogen for this Collagen IV antibody (100% homologous in human, mouse and rat) . This amino acid sequence is from the Canstatin fragment of the protein.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized Collagen IV antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Collagen IV antibody is available for research use only.

Storage Conditions

The lyophilized Collagen IV antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the Collagen IV antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human breast cancer tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to staining.