CaMKK Antibody

Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+) /calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. CaMKK binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+) -independent activity. It catalyzed a 10-fold increase in the total activity of CaMK1 and had no effect on CaMK2. Cotransfection of COS-7 cells with CaMKK and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that CaMKK enhances Camk4-mediated transcriptional regulation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q8VBY2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 10-31 (QDPRAELVDRVAAINVAHLEEA-mouse) were used as the immunogen for this CaMKK antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the CaMKK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CaMKK antibody is available for research use only.

Storage Conditions

After reconstitution, the CaMKK antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CaMKK antibody may be required due to differences in protocols and secondary/substrate sensitivity.