IGFBP3 Antibody

Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P17936

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An amino acid sequence from the middle region of human IGFBP3 (IKKGHAKDSQRYKVDYE) was used as the immunogen for this IGFBP3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the IGFBP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IGFBP3 antibody is available for research use only.

Storage Conditions

After reconstitution, the IGFBP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the IGFBP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Secreted

Image Legend

Western blot testing of IGFBP3 antibody and Lane 1: 293T; 2: MCF- 7; 3: A549; 4: SW620. Expected molecular weight: ~31/40-44 kDa (unmodified/glycosylated) .