Lipoamide Dehydrogenase Antibody

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD) . DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P09622

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Lipoamide Dehydrogenase (EAFREANLAASFGKSINF) was used as the immunogen for this Lipoamide Dehydrogenase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Lipoamide Dehydrogenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Lipoamide Dehydrogenase antibody is available for research use only.

Storage Conditions

After reconstitution, the Lipoamide Dehydrogenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Lipoamide Dehydrogenase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE rat liver tissue with Lipoamide Dehydrogenase antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.