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GM-CSF Antibody

Granulocyte-macrophage colony-stimulating factor is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. It stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P04141

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the middle region of human GMCSF (MFDLQEPTCLQTRLELYKQ) was used as the immunogen for this GM-CSF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the GM-CSF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GM-CSF antibody is available for research use only.

Storage Conditions

After reconstitution, the GM-CSF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the GM-CSF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of GM-CSF antibody and Lane 1: Jurkat; 2: HeLa; 3: HT1080; The protein is visualized between 14~35KD depending on glycosylation.

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