CD14 Antibody

CD14, Cluster of differentiation 14, single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P10810

Host

Rabbit

Reactivity

Mouse

Immunogen

An amino acid sequence from the middle region of mouse CD14 (TRDAWLAELQQWLK) was used as the immunogen for this CD14 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the CD14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD14 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.