Caspase-6 Antibody

Caspase-6 is an enzyme that in humans is encoded by the CASP6 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease family. Using radiation hybrid mapping, the CASP6 gene was localized to human chromosome 4q25-q26. It functions as a downstream enzyme in the caspase activation cascade, and can cleave Lamin A to its signature apoptotic fragment.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

O35397

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids 7-27 (FYRSREVLDPAEQYKMDHKRR-rat) were used as the immunogen for this Caspase-6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Caspase-6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-6 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of Caspase-6 antibody and Lane 1: rat liver; 2: rat kidney; 3: rat testis; 4: NRK; 5: mouse liver; 6: mouse kidney; 7: mouse testis tissue lysate. Predicted molecular weight: ~33 kDa (precursor) .