Caspase-1 Antibody (p20 subunit)

Caspase-1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL-18, and IL-33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P29452

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of mouse CASP1 (LEKAQKLWKENPSEIYPIMNT) was used as the immunogen for this Caspase-1 antibody. This sequence is from the p20 subunit.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Caspase-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) rat thymus, 2) rat C6 and 3) mouse thymus tissue lysate with Caspase-1 antibody. Predicted molecular weight ~45 kDa (full length) .