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Caspase-1 Antibody (p20 subunit)

Caspase-1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, interleukin 18, and interleukin 33 precursor proteins. The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P29466

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the N-terminus of human CASP1 (CSLEEAQRIWKQKSAEIY) was used as the immunogen for this Caspase-1 antibody. The sequence is from the large subunit.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized Caspase-1 antibody can be stored at 4oC. After reconstitution, aliquot and store at -20oC. Repeated freeze/thaw steps should be avoided.

Limitations

This Caspase-1 antibody is available for research use only.

Storage Conditions

The lyophilized Caspase-1 antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Repeated freeze/thaw steps should be avoided.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) Jurkat, 2) Raji and 3) CEM lysate with Caspase-1 antibody at 0.5ug/ml.

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