Annexin VI Antibody

Annexin VI (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca (2+) -dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization.The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal.Ca (2+) -dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

P08133

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Annexin VI (NIRREFIEKYDKSLHQA) was used as the immunogen for this Annexin VI antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Annexin VI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Annexin VI antibody is available for research use only.

Storage Conditions

After reconstitution, the Annexin VI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Annexin VI antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Annexin VI antibody and Lane 1: rat ovary; 2: rat liver; 3: rat intestinum tenue; 4: rat brain; 5: human A549; 6: human Jurkat; 7: human Raji; 8: human CCRF-CEM; 9: human SMMC-7721 cell lysate. Expected molecular weight: 67-76 kDa.