ANGPTL4 Antibody

Angiopoietin-related protein 4 is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, the gene was mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q9BY76

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human ANGPTL4 (WWFGTCSHSNLNGQYFRSI) was used as the immunogen for this ANGPTL4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the ANGPTL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ANGPTL4 antibody is available for research use only.

Storage Conditions

After reconstitution, the ANGPTL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the ANGPTL4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of ANGPTL4 antibody and Lane 1: human placenta; 2: SMMC-7721; 3: HeLa lysate. Predicted molecular weight: ~45 kDa and ~41 kDa, but may be observed at higher molecular weights due to glycosylation.