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Ubiquitin Antibody

Ubiquitin (originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

UniProt

P0CG47

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 29-45 (KIQDKEGIPPDQQRLIF) were used as the immunogen for this Ubiquitin antibody (100% human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Ubiquitin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ubiquitin antibody is available for research use only.

Storage Conditions

After reconstitution, the Ubiquitin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Ubiquitin antibody and Lane 1: rat thymus; 2: human MCF-7; 3: MM231; 4: HeLa; 5: SMMC-7721 cell lysate.

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