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Thrombospondin 2 Antibody / THBS2

Thrombospondin-2 (THBS2) is a protein that in humans is encoded by the THBS2 gene. The protein encoded by this gene belongs to the thrombospondin family. The THBS2 is mapped to 6q27 and it is located on chromosome 17. The gene was transcribed in fibroblasts, smooth muscle cells, and an osteosarcoma cell line. It functions as a protein inhibitor of tumor growth and angiogenesis and modulates the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P35442

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the middle region of human Thrombospondin 2 (EAAKTEKQVCEPENPCKDK) was used as the immunogen for this Thrombospondin 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Thrombospondin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Thrombospondin 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Thrombospondin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

https://www.nsjbio.com/tds-pdf/thrombospondin-2-antibody-thbs2-r30487

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Thrombospondin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of human 1) HeLa, 2) MCF7, 3) U-87 MG, and 4) U-251 cell lysates using Thrombospondin 2 antibody. The expected molecular weight for full-length THBS2 is approximately 130 kDa, but literature reports fully glycosylated forms migrating at 140-170 kDa. In this blot, the major band appears near ~160 kDa, consistent with mature glycosylated THBS2. A secondary band at ~90 kDa corresponds to documented proteolytic fragments described for this extracellular matrix protein.
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