PI3K Antibody (p85 alpha) / PI3-kinase subunit p85 alpha
Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. The PI3K p85 subunit family has two closely related members, alpha and beta. Both cDNAs encode p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P27986
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 65-80 (ERGDFPGTYVEYIGRK-alpha subunit specific) was used as the immunogen for this PI3K antibody (100% human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Store the lyophilized PI3K antibody at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PI3K antibody is available for research use only.
Storage Conditions
Store the lyophilized PI3K antibody at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the PI3K antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of PI3K antibody and human lysates from 1) MCF-7, 2) HeLa, 3) COLO-320 and 4) SW620 cells. Predicted molecular weight ~85 kDa.
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