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CX3CL1 Antibody

Chemokine (C-X3-C motif) ligand 1 is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice) . Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

O35188

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of mouse CX3CL1 (CADPKEKWVQDAMKHLDHQ) was used as the immunogen for this CX3CL1 antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the CX3CL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CX3CL1 antibody is available for research use only.

Storage Conditions

After reconstitution, the CX3CL1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CX3CL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of CX3CL1 antibody and Lane 1: recombinant mouse protein 10ng; 2: 5ng; 3: 2.5ng

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