TNF beta Antibody

Lymphotoxin (previously known as tumor necrosis factor-beta) is a lymphokine cytokine. It is a protein that is produced by Th1 type T-cells and induces vascular endothelial cells to change their surface adhesion molecules to allow phagocytic cells to bind to them. Lymphotoxin is homologous to Tumor Necrosis Factor beta, but secreted by T-cells. It is paracrine due to the small amounts produced. The effects are similar to TNF-alpha, but TNF-beta is also important for the development of lymphoid organs. Nedwin et al. (1985) found that TNFA and TNFB are closely linked on chromosome 6. Study of hybrid cells made with rearranged human chromosome 6 showed that both TNFA and TNFB map to the 6p23-q12 segment.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P01374

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the middle region of human TNF beta (KQNSLLWRANTDRAFL) was used as the immunogen for this TNF beta antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the TNF beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TNF beta antibody is available for research use only.

Storage Conditions

After reconstitution, the TNF beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the TNF beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.