Presenilin 2 Antibody / PSEN2

Presenilin-2 is a protein that in humans is encoded by the PSEN2 gene. Kovacs et al. (1996) demonstrated that the expression patterns of PS1 and PS2 in the brain are extremely similar to each other and that messages for both are primarily detectable in neuronal populations. Immunochemical analyses indicated that PS1 and PS2 are similar in size and localize to similar intracellular compartments (endoplasmic reticulum and Golgi complex) . Li et al. (1997) demonstrated that wildtype PS1 and PS2 are localized to the nuclear membrane, its associated interphase kinetochores, and the centrosomes.In melanocytic cells PSEN2 gene expression may be regulated by MITF.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL

UniProt

P49810

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human Presenilin 2 (EDGENTAQWRSQE) was used as the immunogen for this Presenilin 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Presenilin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Presenilin 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Presenilin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Presenilin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Presenilin 2 antibody and Lane 1: rat brain; 2: rat brain; 3: MCF-7; 4: HeLa; 5: SMMC-7721; 6: CEM lysate. Predicted/observed molecular weight: ~50kDa.