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GRP94 Antibody / HSP90B1

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity) . Functions in endoplasmic reticulum associated degradation (ERAD) . Has ATPase activity. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

P14625

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An amino acid sequence from the middle region of human GRP94 (INPRHPLIRDMLRR) was used as the immunogen for this GRP94 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the GRP94 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GRP94 antibody is available for research use only.

Storage Conditions

After reconstitution, the GRP94 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the GRP94 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) A549, 2) HeLa, 3) Jurkat and 4) K562 cell lysate with GRP94 antibody. Predicted molecular weight ~94 kDa.

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