N-Cadherin Antibody / CDH2 / CD325

NCAD is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM) . Human and mouse nucleotide sequences are 96% identical. The mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. The human Ncad gene contains 16 exons and its sequence is highly similar to both the mouse gene (including the large first and second introns) and other cadherin genes. Cadherins regulates dendritic spine morphogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P19022

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 701-714 (CQCDSNGDCTDVDR) were used as the immunogen for this N-Cadherin antibody (100% homologous human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the N-Cadherin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This N-Cadherin antibody is available for research use only.

Storage Conditions

After reconstitution, the N-Cadherin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the N-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human colon cancer with N-Cadherin antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA for 20 min.