Cyclin D1 Antibody

D-type cyclins are regarded as essential links between cell environment and the core cell cycle machinery. Cyclin D1, the product of the human gene CCND1, is also known as BCL1. Cyclin D1 expression is regulated by the retinoblastoma protein. Expression of Cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. The CCND1 gene can function as an oncogene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P25322

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 219-238 (SPNNFLSCYRTTHFLSRVIK) were used as the immunogen for this Cyclin D1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Cyclin D1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cyclin D1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cyclin D1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Cyclin D1 antibody and Lane 1: rat testis; 2: rat ovary; 3: rat brain; 4: HeLa; 5: MM231; 6: SW620 cell lysate. Predicted molecular weight: 32-36 kDa.