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Bcl-XS Antibody

Bcl-XS is the second mRNA species of Bcl-X protein, which encodes a protein that inhibits the ability of Bcl-2 to enhance the survival of growth factor-deprived cells. In vivo, Bcl-XS mRNA is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Apoptosis was associated with increased protein expression of Bcl-XS, an early marker of apoptosis, and release of Cytochrome C into the cytosol of infected HB cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q07817

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 120-130 (YQSFEQDTFVE-human) were used as the immunogen for this Bcl-XS antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Bcl-XS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Bcl-XS antibody is available for research use only.

Storage Conditions

After reconstitution, the Bcl-XS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Bcl-XS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human HeLa and 2) rat PC-12 cell lysate with Bcl-XS antibody. Predicted/observed molecular weight: ~19 kDa.

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