NOTCH1 Antibody

Notch proteins are single-pass transmembrane receptors that regulate cell fate decisions during development. The Notch family includes 4 receptors, NOTCH1, NOTCH2, NOTCH3, and NOTCH4, whose ligands include JAG1, JAG2, DLL1), DLL3, and DLL4. Notch homolog 1, translocation-associated (NOTCH1), is a human gene encoding a single-pass transmembrane receptor. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. NOTCH1 may normally coordinates the process of somitogenesis, and the activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P46531

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the middle region of human NOTCH1 (QHSYSSPVDNTPSHQLQVPE) was used as the immunogen for this NOTCH1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Notch1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NOTCH1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NOTCH1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NOTCH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of NOTCH1 antibody and Lane 1: MCF-7; 2: HeLa; 3: Jurkat; 4: COLO320. Predicted molecular weight: ~ 270 kDa (full length), ~ 120 kDa (transmembrane fragment) .