Beta Catenin Antibody

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. Beta-catenin binds the cytoplasmic domain of some cadherins. It is an adherens junction protein that plays an important role in various aspects of liver biology.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL

UniProt

P35222

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 764-781 (DGLPPGDSNQLAWFDTDL) were used as the immunogen for this beta Catenin antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the pAb can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This beta Catenin antibody is available for research use only.

Storage Conditions

After reconstitution, the pAb can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human A431 cells with beta Catenin antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.