P65 Antibody NF-kB
The p65 (RELA) heterodimer is the most abundant form of NF-KB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A (PKA) . The transcriptional coactivator CBP/p300 associates with NF-kappa-B through 2 sites, an N-terminal domain that interacts with the C-terminal region of the unphosphorylated protein, and a second domain that only interacts with p65 phosphorylated on serine-276.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q04206
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Limitations
This p65 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of p65 antibody and Lane 1: human colon cancer; 2: HeLa lysate. Expected molecular weight ~65 kDa.
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