Caspase-2 Antibody

Caspase-2/CASP2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. Activation of Caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of Caspase-2 and sensitization to apoptosis by genotoxic stimuli. CASP2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P42575

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 417-439 (REGYAPGTEFHRCKEMSEYCSTL) were used as the immunogen for this Caspase-2 antibody (100% homologous in human, mouse and rat) . This sequence is from the small subunit.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Caspase-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Caspase-2 antibody and Lane 1: human CCRF-CEM; 2: human SMMC-7721 cell lysate. Predicted molecular weight: ~47 kDa (full), ~31 kDa (large+small subunit), ~11 kDa (small subunit) .